3ZOM
M.acetivorans protoglobin F145W mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.974, 48.147, 81.001 |
| Unit cell angles | 90.00, 95.06, 90.00 |
Refinement procedure
| Resolution | 80.580 - 2.200 |
| R-factor | 0.19033 |
| Rwork | 0.188 |
| R-free | 0.23290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2veb |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.279 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.580 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.130 | 0.360 |
| Number of reflections | 20117 | |
| <I/σ(I)> | 15.9 | 5.4 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 6.7 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 20% PEG 4000, 10% ISOPROPANOL, 0.1 M HEPES PH 7.5, 0.02 M POTASSIUM FERRICYANIDE, 0.01 M KCN |
