3ZO6
Crystal structure of Bacillus pseudofirmus OF4 mutant ATP synthase c12 ring.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-04-02 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 90.220, 114.550, 137.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.354 - 4.104 |
| R-factor | 0.2777 |
| Rwork | 0.275 |
| R-free | 0.33540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x2v |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.072 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 4.200 |
| High resolution limit [Å] | 4.100 | 4.100 |
| Rmerge | 0.400 | |
| Number of reflections | 11501 | |
| <I/σ(I)> | 7.42 | 1.3 |
| Completeness [%] | 98.4 | 81 |
| Redundancy | 3.57 | 3.33 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | pH 9.0 |
