3ZO5
Structure of SENP2-Loop1 in complex with preSUMO-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | H 3 2 |
Unit cell lengths | 145.518, 145.518, 104.671 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 80.520 - 2.150 |
R-factor | 0.19041 |
Rwork | 0.188 |
R-free | 0.23627 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2io0 |
RMSD bond length | 0.020 |
RMSD bond angle | 2.337 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.330 | 2.260 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.040 | 0.770 |
Number of reflections | 23228 | |
<I/σ(I)> | 21 | 2.2 |
Completeness [%] | 99.8 | 99.2 |
Redundancy | 5.1 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |