3ZNZ
Crystal structure of OTULIN OTU domain (C129A) in complex with Met1- di ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Collection date | 2012-01-29 |
| Detector | ADSC CCD |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 100.020, 100.020, 280.260 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.802 - 1.900 |
| R-factor | 0.1857 |
| Rwork | 0.184 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3znv |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.018 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.800 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.090 | 0.780 |
| Number of reflections | 42974 | |
| <I/σ(I)> | 14.4 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.9 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 100 MM BIS-TRIS, 2M (NH4)2SO4, PH 6.5 |
