3ZNQ
IN VITRO AND IN VIVO INHIBITION OF HUMAN D-AMINO ACID OXIDASE: REGULATION OF D-SERINE CONCENTRATION IN THE BRAIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 84.471, 84.471, 188.672 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.650 - 2.750 |
| R-factor | 0.2018 |
| Rwork | 0.199 |
| R-free | 0.25520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2du8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.050 |
| Data reduction software | HKL |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.140 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.110 | 0.540 |
| Number of reflections | 18932 | |
| <I/σ(I)> | 12.5 | 1.5 |
| Completeness [%] | 90.4 | 75 |
| Redundancy | 3.85 | 3.85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
