3ZNL
H5 Haemagglutinin in Complex with 6-O-Sulfo-Sialyl-Lewis X (Sulfated Lewis X)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-04-23 |
Detector | DECTRIS PILATUS 2M |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 175.980, 101.600, 161.370 |
Unit cell angles | 90.00, 111.32, 90.00 |
Refinement procedure
Resolution | 35.700 - 2.500 |
R-factor | 0.23789 |
Rwork | 0.237 |
R-free | 0.25582 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ibx |
RMSD bond length | 0.005 |
RMSD bond angle | 1.008 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 150.760 |
High resolution limit [Å] | 2.500 |
Rmerge | 0.110 |
Number of reflections | 64504 |
<I/σ(I)> | 7.3 |
Completeness [%] | 70.4 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.1 M HEPES PH 7.0, 0.05 M MGCL2, 28 - 30 % PEG 550 MME |