3ZN6

VP16-VP17 complex, a complex of the two major capsid proteins of bacteriophage P23-77

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-10-10
Detector	DECTRIS PILATUS 6M
Spacegroup name	C 1 2 1
Unit cell lengths	76.780, 69.610, 81.620
Unit cell angles	90.00, 104.99, 90.00

Refinement procedure

Resolution	22.400 - 1.530
R-factor	0.173
Rwork	0.172
R-free	0.19740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 3ZMO AND 3ZMN
RMSD bond length	0.010
RMSD bond angle	1.070
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	BUSTER (2.11.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.600	1.570
High resolution limit [Å]	1.530	1.530
Rmerge	0.060	1.020
Number of reflections	61458
<I/σ(I)>	17.7	2.9
Completeness [%]	98.4	83.3
Redundancy	6.3	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7		SITTING DROP VAPOUR DIFFUSION SYSTEM IN A 96-WELL PLATE, 100 NL OF PROTEIN MIXTURE CONSISTING OF VP16 AND VP17 (1.7 MG/ML AND 2 MG/ML, RESPECTIVELY, IN 20 MM TRIS-BUFFER PH 7.4) MIXED IN 1:1 MOLAR RATIO, WAS MIXED WITH 100 NL SOLUTION CONSISTING OF 1.1 M DIAMMONIUM TARTRATE PH 7. ONE WELL-DIFFRACTING CRYSTAL THAT WAS USED FOR DATA COLLECTION TOOK THREE MONTHS TO GROW.