3ZN5
VP16, a capsid protein of bacteriophage P23-77 (VP16-virus-derived)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-01-28 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.410, 77.050, 403.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.160 - 2.360 |
R-factor | 0.1946 |
Rwork | 0.193 |
R-free | 0.23440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB CODE 3ZMO |
RMSD bond length | 0.010 |
RMSD bond angle | 1.070 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.160 | 2.420 |
High resolution limit [Å] | 2.360 | 2.360 |
Rmerge | 0.110 | 1.140 |
Number of reflections | 54151 | |
<I/σ(I)> | 7.5 | 1.3 |
Completeness [%] | 99.1 | 99.6 |
Redundancy | 3.2 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | SITTING DROP VAPOUR DIFFUSION SYSTEM, MICROLITER DROPS OF PURIFIED P23-77 VIRION (PROTEIN CONCENTRATION 2.4 MG/ML, SAMPLE BUFFER 20 MM TRIS-HCL PH 7.5, 5 MM MGCL2 AND 150 MM NACL) MIXED 1:1 WITH SOLUTION CONSISTING OF 0.1 M CITRIC ACID PH 3.5, 20 MM TRIS-HCL PH 7.5, 5 MM MGCL2, 150 MM NACL AND 25%(W/V) PEG 3350. |