3ZN4
VP16, a capsid protein of bacteriophage P23-77 (VP16-type-2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-26 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.600, 68.570, 31.580 |
| Unit cell angles | 90.00, 96.44, 90.00 |
Refinement procedure
| Resolution | 17.790 - 1.260 |
| R-factor | 0.1577 |
| Rwork | 0.156 |
| R-free | 0.18400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zmo |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.300 | 1.300 |
| High resolution limit [Å] | 1.260 | 1.260 |
| Rmerge | 0.050 | 0.630 |
| Number of reflections | 37146 | |
| <I/σ(I)> | 23.1 | 2.6 |
| Completeness [%] | 85.8 | 41.4 |
| Redundancy | 7.5 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | SITTING DROP VAPOUR DIFFUSION SYSTEM IN A 96-WELL PLATE, 200 NL OF PROTEIN (2-3 MG/ML IN 20 MM TRIS-BUFFER PH 7.4) MIXED WITH 200 NL SOLUTION CONSISTING 20%(W/V) PEG6000 AND 0.1 M CITRATE PH 4. |
