3ZN3
N-terminal domain of S. pombe Cdc23 APC subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 56.875, 56.875, 154.814 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 154.810 - 1.900 |
| R-factor | 0.20727 |
| Rwork | 0.206 |
| R-free | 0.25883 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.006 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEIT |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.830 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.110 | 0.690 |
| Number of reflections | 20943 | |
| <I/σ(I)> | 18.8 | 4.8 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 23.7 | 18.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 200 MM SODIUM FORMATE, PH 7.5, 20% (W/V) PEG 3350 |
