3ZMO
VP16, a capsid protein of bacteriophage P23-77 (VP16-type-1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-12 |
| Detector | ADSC CCD |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 61.850, 61.850, 251.220 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 53.560 - 1.800 |
| R-factor | 0.1898 |
| Rwork | 0.189 |
| R-free | 0.21480 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | xia2 |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.800 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.080 | 1.040 |
| Number of reflections | 27581 | |
| <I/σ(I)> | 30.7 | 3.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 28.5 | 21 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | SITTING DROP VAPOUR DIFFUSION SYSTEM, MICROLITER DROPS OF PROTEIN (2-3 MG/ML IN 20 MM TRIS-BUFFER PH 7.4) MIXED 1:1 WITH A SOLUTION CONSISTING 5%(W/V) PEG1000 AND 5%(W/V) PEG8000 (DISSOLVED IN AUTOCLAVED WATER). |
