3ZMN

VP17, a capsid protein of bacteriophage P23-77

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-26
Detector	ADSC CCD
Spacegroup name	P 61 2 2
Unit cell lengths	107.210, 107.210, 233.780
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.940 - 2.260
R-factor	0.1868
Rwork	0.185
R-free	0.21320
Structure solution method	SIRAS
Starting model (for MR)	NONE
RMSD bond length	0.010
RMSD bond angle	1.140
Data scaling software	xia2
Phasing software	xia2
Refinement software	BUSTER (2.11.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.700	2.320
High resolution limit [Å]	2.260	2.260
Rmerge	0.080	0.920
Number of reflections	37893
<I/σ(I)>	38.6	5.3
Completeness [%]	100.0	100
Redundancy	35.5	36.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7		SITTING DROP VAPOUR DIFFUSION SYSTEM, MICROLITER DROPS OF PROTEIN(2-3 MG/ML IN 20 MM TRIS-BUFFER PH 7.4) MIXED 1:1 WITH SOLUTION CONSISTING OF 1.9 M SODIUM FORMATE AND 0.1 M BIS-TRIS BUFFER PH 7.