3ZMH
Structure of E.coli rhomboid protease GlpG in complex with monobactam L62
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-08-08 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | H 3 2 |
Unit cell lengths | 110.150, 110.150, 128.630 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 76.622 - 2.300 |
R-factor | 0.19 |
Rwork | 0.188 |
R-free | 0.22880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xov |
RMSD bond length | 0.007 |
RMSD bond angle | 1.022 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 76.620 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.110 | 0.680 |
Number of reflections | 13547 | |
<I/σ(I)> | 10.9 | 2.6 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 5.9 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 2M NACL, 0.1M BIS-TRIS PH7.0, 298K |