3ZMF
Salmonella enterica SadA 303-358 fused to GCN4 adaptors (SadAK2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-31 |
| Detector | MARRESEARCH |
| Spacegroup name | P 3 |
| Unit cell lengths | 46.590, 46.590, 128.980 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.510 - 1.850 |
| R-factor | 0.18325 |
| Rwork | 0.181 |
| R-free | 0.22051 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gcm |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.147 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.500 | 1.960 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.060 | 0.590 |
| Number of reflections | 26591 | |
| <I/σ(I)> | 14.2 | 1.9 |
| Completeness [%] | 99.2 | 97.4 |
| Redundancy | 3.72 | 3.15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 50% PEG 200, 0.1 M NA-CITRATE PH 5.5 |
