3ZMC
Native structure of Farnesyl Pyrophosphate Synthase from Pseudomonas aeruginosa PA01, with bound substrate molecule Geranyl pyrophosphate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-29 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 85.963, 98.778, 130.554 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.860 - 1.870 |
| R-factor | 0.20711 |
| Rwork | 0.205 |
| R-free | 0.25050 |
| Structure solution method | SIR |
| Starting model (for MR) | 3zcd |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.864 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.830 | 1.910 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.110 | 0.650 |
| Number of reflections | 46143 | |
| <I/σ(I)> | 9.2 | 1.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.15 M MGCL2, 20% PEG8000, 0.1 M TRISCL PH 8 |
