3ZM8
Crystal structure of Podospora anserina GH26-CBM35 beta-(1,4)- mannanase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-07 |
| Detector | ADSC CCD |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 97.490, 97.490, 268.717 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.300 - 2.850 |
| R-factor | 0.2088 |
| Rwork | 0.206 |
| R-free | 0.25730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2QHA 2bvt 2VX4 AND 2WHK |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.040 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.080 | 0.650 |
| Number of reflections | 18575 | |
| <I/σ(I)> | 25.6 | 4.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.8 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1 M TRIS PH 7.0, 0.2 M NACL, 0.8 M POTASSIUM SODIUM TARTRATE, 1 MM HGCL2 |
