3ZM7
CRYSTAL STRUCTURE OF THE ATPASE REGION OF Mycobacterium tuberculosis GyrB WITH AMPPCP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.615, 171.916, 109.230 |
| Unit cell angles | 90.00, 110.22, 90.00 |
Refinement procedure
| Resolution | 25.000 - 3.300 |
| R-factor | 0.197 |
| Rwork | 0.196 |
| R-free | 0.22310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zkb |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.190 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.500 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.060 | 0.620 |
| Number of reflections | 41737 | |
| <I/σ(I)> | 16.5 | 2.4 |
| Completeness [%] | 98.8 | 95.5 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 24% PEG-1500, 0.1 M MES PH 6.5, 5 MM MGCL2, AND 1.2% MYO-INOSITOL |
