3ZKW
Periplasmic Binding Protein CeuE apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-07 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.950, 62.740, 67.980 |
| Unit cell angles | 82.19, 76.74, 75.96 |
Refinement procedure
| Resolution | 65.930 - 1.710 |
| R-factor | 0.19699 |
| Rwork | 0.195 |
| R-free | 0.22743 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2chu |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.064 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.700 | 1.740 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.040 | 0.450 |
| Number of reflections | 95173 | |
| <I/σ(I)> | 11.1 | 1.9 |
| Completeness [%] | 96.8 | 95.2 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 0.1 M MIB (SODIUM MALONATE, IMIDAZOLE, BORIC ACID) BUFFER, PH 5, 25% (W/V) PEG 1500 |
