3ZKD
CRYSTAL STRUCTURE OF THE ATPASE REGION OF Mycobacterium tuberculosis GyrB WITH AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 |
| Unit cell lengths | 71.953, 97.851, 138.075 |
| Unit cell angles | 108.21, 105.18, 91.04 |
Refinement procedure
| Resolution | 24.810 - 2.950 |
| R-factor | 0.1847 |
| Rwork | 0.182 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ei1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.160 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.000 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.080 | 0.270 |
| Number of reflections | 64548 | |
| <I/σ(I)> | 10.8 | 1.9 |
| Completeness [%] | 84.5 | 86.2 |
| Redundancy | 1.68 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
