3ZKC
Crystal structure of the master regulator for biofilm formation SinR in complex with DNA.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-04 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 65.364, 79.739, 67.939 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.556 - 3.000 |
| R-factor | 0.2427 |
| Rwork | 0.242 |
| R-free | 0.26430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b0n |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.788 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.500 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.030 | 0.670 |
| Number of reflections | 13020 | |
| <I/σ(I)> | 21.3 | 2.3 |
| Completeness [%] | 98.4 | 98.7 |
| Redundancy | 3.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 0.01M ZNCL2, 0.1M SODIUM ACTEATE, PH 5.0, 20% (W/V) PEG 6000 |
