3ZK1
Crystal structure of the sodium binding rotor ring at pH 5.3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-08-15 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 135.770, 83.900, 150.990 |
| Unit cell angles | 90.00, 112.85, 90.00 |
Refinement procedure
| Resolution | 29.747 - 2.200 |
| R-factor | 0.209 |
| Rwork | 0.208 |
| R-free | 0.23370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wgm |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.097 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.210 | 1.150 |
| Number of reflections | 158380 | |
| <I/σ(I)> | 8.65 | 1.35 |
| Completeness [%] | 99.5 | 99.4 |
| Redundancy | 4.5 | 4.31 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.3 | pH 5.3 |
