3ZJO
M.acetivorans protoglobin in complex with azide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.880, 48.820, 80.590 |
| Unit cell angles | 90.00, 122.60, 90.00 |
Refinement procedure
| Resolution | 32.910 - 1.800 |
| R-factor | 0.19256 |
| Rwork | 0.189 |
| R-free | 0.25627 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2veb |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.447 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.620 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.080 | 0.400 |
| Number of reflections | 35389 | |
| <I/σ(I)> | 8.4 | 3.4 |
| Completeness [%] | 97.1 | 97.5 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 30% PEG 4000, 0.2 M LITHIUM SULPHATE, 0.1 M HEPES PH 7.5, 0.01 M POTASSIUM FERRICYANIDE, 0.1 M SODIUM AZIDE |
