3ZJB
The structure of the TRAF domain of human TRAF4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-30 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 54.620, 85.440, 61.650 |
Unit cell angles | 90.00, 108.08, 90.00 |
Refinement procedure
Resolution | 58.600 - 1.840 |
R-factor | 0.165 |
Rwork | 0.163 |
R-free | 0.19950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ca9 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.040 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.600 | 1.880 |
High resolution limit [Å] | 1.850 | 1.840 |
Rmerge | 0.080 | 1.290 |
Number of reflections | 46352 | |
<I/σ(I)> | 10.4 | 1.04 |
Completeness [%] | 97.3 | 73.9 |
Redundancy | 3.6 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 15% PEG 4000, 0.1M HEPES PH 7.0 |