3ZJA
The crystal structure of a Cu(I) metallochaperone from Streptomyces lividans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-25 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.480, 47.840, 50.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.320 - 1.480 |
| R-factor | 0.18741 |
| Rwork | 0.185 |
| R-free | 0.22675 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.800 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.600 | 1.560 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.060 | 0.480 |
| Number of reflections | 17951 | |
| <I/σ(I)> | 9.5 | 2.2 |
| Completeness [%] | 98.1 | 98.8 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 30% PEG-MME2000, 0.1M KSCN |
