3ZJ4
Neurospora Crassa Catalase-3 expressed in E. coli, triclinic form.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-10 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 1 |
| Unit cell lengths | 85.445, 88.207, 104.398 |
| Unit cell angles | 82.08, 82.48, 62.33 |
Refinement procedure
| Resolution | 29.399 - 3.098 |
| R-factor | 0.1994 |
| Rwork | 0.197 |
| R-free | 0.24550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ej6 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.864 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.500 | 3.260 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.170 | 0.510 |
| Number of reflections | 47857 | |
| <I/σ(I)> | 8.5 | 2.8 |
| Completeness [%] | 98.6 | 97.1 |
| Redundancy | 4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 100MM HEPES PH 7.5, 20 PERCENT PEG 10,000 |
