3ZIW
Clostridium perfringens enterotoxin, D48A mutation and N-terminal 37 residues deleted
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-29 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 190.660, 128.020, 136.430 |
| Unit cell angles | 90.00, 133.81, 90.00 |
Refinement procedure
| Resolution | 49.230 - 1.900 |
| R-factor | 0.176 |
| Rwork | 0.175 |
| R-free | 0.19560 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.150 | 0.860 |
| Number of reflections | 186226 | |
| <I/σ(I)> | 7.42 | 1.26 |
| Completeness [%] | 99.1 | 93.1 |
| Redundancy | 7.3 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 25 % PEG 1500, 0.1 M SPG BUFFER, PH 6.0 |
