3ZIV
Crystal Structure of Ancestral Thioredoxin Relative to last Archaea- Eukaryotes Common Ancestor (AECA) from the Precambrian Period
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.650, 48.850, 91.170 |
| Unit cell angles | 90.00, 93.23, 90.00 |
Refinement procedure
| Resolution | 45.512 - 2.650 |
| R-factor | 0.1852 |
| Rwork | 0.181 |
| R-free | 0.27620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ynx |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.150 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.510 | 2.740 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.060 | 0.600 |
| Number of reflections | 9592 | |
| <I/σ(I)> | 16.21 | 3.24 |
| Completeness [%] | 97.9 | 97.9 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | COUNTER-DIFFUSION | 7 | 277 | COUNTER DIFFUSION, 25% PEG1500, PH 9.0, 277K |
