3ZIR
minor-site specific NLS (B141)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 300 |
| Collection date | 2010-09-02 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.000, 90.340, 100.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.870 - 2.300 |
| R-factor | 0.1767 |
| Rwork | 0.175 |
| R-free | 0.20190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ial |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.960 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.930 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.130 | 0.880 |
| Number of reflections | 36861 | |
| <I/σ(I)> | 10.3 | 2.3 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 7.1 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.75 M SODIUM CITRATE, 0.1 M HEPES BUFFER (PH 6.5) AND 10 MM DTT |
