3ZIH
Bacillus subtilis SepF, C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-15 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 40.456, 40.456, 170.331 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.18761 |
| Rwork | 0.184 |
| R-free | 0.24596 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.815 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.580 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.090 | 0.420 |
| Number of reflections | 13575 | |
| <I/σ(I)> | 14.5 | 4.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 9.6 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 30% PEG 8000, 0.2 M AMMONIUM SULPHATE |
