3ZHQ
Crystal structure of the H747A mutant of the SucA domain of Mycobacterium smegmatis KGD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-02-26 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 80.675, 83.737, 159.577 |
| Unit cell angles | 99.86, 98.95, 100.44 |
Refinement procedure
| Resolution | 48.970 - 2.500 |
| R-factor | 0.1952 |
| Rwork | 0.194 |
| R-free | 0.22340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yic |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.970 | 2.630 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.110 | 0.560 |
| Number of reflections | 133094 | |
| <I/σ(I)> | 9.1 | 2.3 |
| Completeness [%] | 96.6 | 94.4 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.6 | 52% MPD, 20 MM NA ACETATE, pH 7.6 |
