3ZHK
The crystal structure of single domain antibody 2x1 scaffold
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2012-07-06 |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 104.415, 55.701, 74.539 |
Unit cell angles | 90.00, 126.44, 90.00 |
Refinement procedure
Resolution | 31.975 - 1.962 |
R-factor | 0.2552 |
Rwork | 0.253 |
R-free | 0.29770 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.186 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.990 |
High resolution limit [Å] | 1.960 | 1.960 |
Rmerge | 0.100 | 0.310 |
Number of reflections | 24632 | |
<I/σ(I)> | 39.1 | 9.3 |
Completeness [%] | 99.2 | 92.5 |
Redundancy | 6.9 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 37% PEG8K, 0.2M AMMONIUM SULFATE |