3ZHE
Structure of the C. elegans SMG5-SMG7 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-18 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 248.160, 82.000, 154.510 |
| Unit cell angles | 90.00, 116.88, 90.00 |
Refinement procedure
| Resolution | 49.337 - 3.000 |
| R-factor | 0.2232 |
| Rwork | 0.222 |
| R-free | 0.24870 |
| Structure solution method | MIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.685 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.200 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 55840 | |
| <I/σ(I)> | 14.5 | 2.37 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 6.8 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 0.1 M SODIUM CITRATE (PH 5.0), 8% (W/V) PEG 8000, 10% GLYCEROL, 500 MM NACL, 60 MM (NH4)2SO4, 4 MM DTT AND 2 MM TCEP |
