3ZHC
Structure of the phytase from Citrobacter braakii at 2.3 angstrom resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 121.510, 121.510, 129.110 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.080 - 2.300 |
| R-factor | 0.19694 |
| Rwork | 0.195 |
| R-free | 0.23726 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.356 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.010 |
| Number of reflections | 49385 |
| <I/σ(I)> | 16.1 |
| Completeness [%] | 100.0 |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 4.0 M SODIUM FORMATE, pH 7.0 |
