3ZH9

Bacillus subtilis DNA clamp loader delta protein (YqeN)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2010-09-25
Detector	ADSC CCD
Spacegroup name	P 43 21 2
Unit cell lengths	81.600, 81.600, 157.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.800 - 2.100
R-factor	0.1938
Rwork	0.192
R-free	0.23890
Structure solution method	MAD
Starting model (for MR)	NONE
RMSD bond length	0.007
RMSD bond angle	0.988
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.700	2.400
High resolution limit [Å]	2.100	2.100
Rmerge	0.090	0.720
Number of reflections	31753
<I/σ(I)>	11.5	2.7
Completeness [%]	99.6	99.9
Redundancy	6.7	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.5		1.0 M AMMONIUM SULPHATE, 0.1 M BIS-TRIS, PH 5.5, 6 % W/V PEG3350