3ZH5
The structure of Haemophilus influenzae protein E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-17 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.187, 57.258, 61.374 |
| Unit cell angles | 90.00, 96.05, 90.00 |
Refinement procedure
| Resolution | 26.930 - 1.801 |
| R-factor | 0.2023 |
| Rwork | 0.201 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.419 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.060 | 0.460 |
| Number of reflections | 115628 | |
| <I/σ(I)> | 12.34 | 2.64 |
| Completeness [%] | 99.5 | 98.6 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM SPG BUFFER PH 6.0, 25% W/V POLYETHYLENE GLYCOL 1500. |
