3ZH2
Structure of Plasmodium falciparum lactate dehydrogenase in complex with a DNA aptamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-17 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.461, 159.489, 88.660 |
| Unit cell angles | 90.00, 102.18, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.20832 |
| Rwork | 0.206 |
| R-free | 0.25664 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ldg |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.109 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.120 | 0.650 |
| Number of reflections | 121507 | |
| <I/σ(I)> | 11.1 | 1.5 |
| Completeness [%] | 98.6 | 94.1 |
| Redundancy | 3.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.2 M KCL, 0.1 M MAGNESIUM ACETATE, 0.05 M SODIUM CACODYLATE PH 6.5, 10% W/V PEG 8000 |
