3ZGX
Crystal structure of the kleisin-N SMC interface in prokaryotic condensin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-10 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 |
| Unit cell lengths | 107.435, 107.435, 102.821 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.968 - 3.400 |
| R-factor | 0.2418 |
| Rwork | 0.243 |
| R-free | 0.27700 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.573 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.970 | 3.580 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.090 | 0.580 |
| Number of reflections | 16156 | |
| <I/σ(I)> | 18.8 | 3.3 |
| Completeness [%] | 99.9 | 99.2 |
| Redundancy | 9.5 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 8 % ISOPROPANOL, 20 MM MAGNESIUM CHLORIDE AND 50 MM MES PH 6.5 |
