3ZGE
Greater efficiency of photosynthetic carbon fixation due to single amino acid substitution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 162.520, 122.110, 131.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.000 - 2.490 |
| R-factor | 0.20425 |
| Rwork | 0.204 |
| R-free | 0.23719 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jqo |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.794 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.830 | 2.620 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Number of reflections | 90203 | |
| <I/σ(I)> | 5.9 | 5.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 26.2 | 24 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 0.2 M AMMONIUM SULFATE, 0.1 M TRI-SODIUM CITRATE/HCL PH 5.6, 15 % PEG 4000 |
