3ZGB

Greater efficiency of photosynthetic carbon fixation due to single amino acid substitution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-27
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 21 21 2
Unit cell lengths	165.549, 121.700, 132.031
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	78.720 - 2.710
R-factor	0.18854
Rwork	0.188
R-free	0.22985
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zbe
RMSD bond length	0.016
RMSD bond angle	1.758
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	165.550	2.860
High resolution limit [Å]	2.710	2.710
Number of reflections	71548
<I/σ(I)>	6.3	2
Completeness [%]	100.0	100
Redundancy	15.8	16.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.6		0.2 M AMMONIUM SULFATE, 0.1 M TRI-SODIUM CITRATE/HCL PH 5.6, 10 % (W/V) PEG 4000