3ZFH
Crystal structure of Pseudomonas aeruginosa inosine 5'-monophosphate dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-01-26 |
| Spacegroup name | I 4 |
| Unit cell lengths | 115.546, 115.546, 56.443 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 81.700 - 2.250 |
| R-factor | 0.15003 |
| Rwork | 0.148 |
| R-free | 0.19024 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tsb |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.522 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.370 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.090 | 0.490 |
| Number of reflections | 16612 | |
| <I/σ(I)> | 16.3 | 4.3 |
| Completeness [%] | 94.6 | 97 |
| Redundancy | 5.2 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M SODIUM ACETATE, 40 % 2-METHYL-2,4-PENTANEDIOL |
