3ZF4
Phage dUTPases control transfer of virulence genes by a proto- oncogenic G protein-like mechanism. (Staphylococcus bacteriophage 80alpha dUTPase Y81A mutant with dUpNHpp).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 87.470, 87.470, 87.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.850 - 3.100 |
| R-factor | 0.24698 |
| Rwork | 0.240 |
| R-free | 0.29059 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zez |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.922 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.900 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.060 | 0.330 |
| Number of reflections | 4224 | |
| <I/σ(I)> | 8.2 | 2.4 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 5.1 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
