3ZF1
Phage dUTPases control transfer of virulence genes by a proto- oncogenic G protein-like mechanism. (Staphylococcus bacteriophage 80alpha dUTPase D81N mutant with dUpNHpp).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 87.210, 87.210, 87.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.350 - 3.000 |
| R-factor | 0.23564 |
| Rwork | 0.230 |
| R-free | 0.28482 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zez |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.961 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.400 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.080 | 0.330 |
| Number of reflections | 4611 | |
| <I/σ(I)> | 7.6 | 2.2 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6.8 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2-8% TERT-BUTANOL, 0.1M TRIS (PH 8.5), 30-50% MPD OR PEG400 |
