3ZF1
Phage dUTPases control transfer of virulence genes by a proto- oncogenic G protein-like mechanism. (Staphylococcus bacteriophage 80alpha dUTPase D81N mutant with dUpNHpp).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 21 3 |
Unit cell lengths | 87.210, 87.210, 87.210 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.350 - 3.000 |
R-factor | 0.23564 |
Rwork | 0.230 |
R-free | 0.28482 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zez |
RMSD bond length | 0.005 |
RMSD bond angle | 0.961 |
Data reduction software | XDS |
Data scaling software | SCALA |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.400 | 3.160 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.080 | 0.330 |
Number of reflections | 4611 | |
<I/σ(I)> | 7.6 | 2.2 |
Completeness [%] | 99.7 | 100 |
Redundancy | 6.8 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 2-8% TERT-BUTANOL, 0.1M TRIS (PH 8.5), 30-50% MPD OR PEG400 |