3ZF0
Phage dUTPases control transfer of virulence genes by a proto-oncogenic G protein-like mechanism. (Staphylococcus bacteriophage 80alpha dUTPase D81A mutant with dUpNHpp).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 21 3 |
Unit cell lengths | 87.120, 87.120, 87.120 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.300 - 2.900 |
R-factor | 0.24255 |
Rwork | 0.242 |
R-free | 0.27463 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.996 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.300 | 3.060 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.070 | 0.350 |
Number of reflections | 5103 | |
<I/σ(I)> | 17.6 | 2.1 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.5 | 8.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 2-8% TERT-BUTANOL, 0.1M TRIS (PH 8.5), 30-50% MPD OR PEG400. |