3ZEK
Hen egg-white lysozyme structure determined at room temperature by in- situ diffraction in ChipX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 298 |
Detector technology | PIXEL |
Collection date | 2012-08-20 |
Detector | DECTRIS PILATUS 2M |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.250, 79.250, 37.970 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.415 - 1.430 |
R-factor | 0.1692 |
Rwork | 0.169 |
R-free | 0.18260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iee |
RMSD bond length | 0.004 |
RMSD bond angle | 0.937 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.520 |
High resolution limit [Å] | 1.430 | 1.430 |
Rmerge | 0.090 | 0.410 |
Number of reflections | 22727 | |
<I/σ(I)> | 17.4 | 3.7 |
Completeness [%] | 98.9 | 97.4 |
Redundancy | 9.4 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | COUNTER-DIFFUSION | 4.5 | 293 | LYSOZYME WAS CRYSTALLIZED AT 293 K BY COUNTER-DIFFUSION IN A CHIPX MICROFLUIDIC DEVICE. MICROFLUIDIC CHANNELS WERE FILLED WITH A 50 MG/ML LYSOZYME SOLUTION CONTAINING 0.3% BETA-OCTYL-GLUCOSIDE (M/V) TO FACILITATE SAMPLE LOADING BY CAPILLARITY. CRYSTALLANT RESERVOIRS WERE FILLED WITH 1 M NACL CONTAINING 30% (M/V) PEG-3350 OR 2 M NACL, AND 0.1 M SODIUM ACETATE PH 4.5. |