3ZE4
Crystal structure of the integral membrane diacylglycerol kinase - wild-type
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-13 |
Detector | MARRESEARCH |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 74.970, 74.970, 196.900 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.226 - 3.702 |
R-factor | 0.2862 |
Rwork | 0.285 |
R-free | 0.31180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ze5 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.795 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.230 | 3.800 |
High resolution limit [Å] | 3.700 | 3.700 |
Rmerge | 0.080 | 0.720 |
Number of reflections | 7069 | |
<I/σ(I)> | 11.8 | 2 |
Completeness [%] | 96.7 | 98 |
Redundancy | 4.8 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 5.6 | 277 | 3-5 %(V/V) 2-METHYL-2, 4-PENTANEDIOL, 0.1 M SODIUM CHLORIDE, 0.1 M LITHIUM NITRATE, 0.1 M SODIUM CITRATE/HCL PH 5.6. CRYSTALLIZED USING THE IN MESO (LIPIDIC CUBIC PHASE) METHOD AT 4 DEGREE CELSIUS WITH THE 7.8 MONOACYLGLYCEROL (7.8 MAG) AS THE HOSTING LIPID. |