3ZDM
Crystal structure of the Sgt2 N domain and the Get5 UBL domain complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 287 |
Detector technology | CCD |
Collection date | 2012-10-15 |
Detector | BRUKER SMART 6500 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 137.109, 34.127, 92.092 |
Unit cell angles | 90.00, 105.84, 90.00 |
Refinement procedure
Resolution | 28.467 - 1.803 |
R-factor | 0.1887 |
Rwork | 0.187 |
R-free | 0.22610 |
Structure solution method | MAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.014 |
RMSD bond angle | 1.343 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.060 | 0.430 |
Number of reflections | 37569 | |
<I/σ(I)> | 27.2 | 4.2 |
Completeness [%] | 97.8 | 97.2 |
Redundancy | 7 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 0.1 M MIB BUFFER, PH 7.0 25% PEG 1500 |