3ZDC
Structure of E. coli ExoIX in complex with the palindromic 5ov4 DNA oligonucleotide, potassium and calcium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-04 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 66.178, 152.688, 34.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.530 - 1.530 |
| R-factor | 0.20603 |
| Rwork | 0.205 |
| R-free | 0.22865 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | EXOIX FLAP1 FRAGMENT COMPLEX STRUCTURE |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.429 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.610 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmerge | 0.060 | 0.420 |
| Number of reflections | 53386 | |
| <I/σ(I)> | 13.7 | 3.2 |
| Completeness [%] | 97.9 | 97.9 |
| Redundancy | 4.2 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.2 M CALCIUM ACETATE, 15% (W/V) PEG-3350, pH 6.5 |
