3ZD4

Full-Length Hammerhead Ribozyme with G12A substitution at the general base position

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Spacegroup name	C 1 2 1
Unit cell lengths	50.140, 68.450, 60.220
Unit cell angles	90.00, 112.56, 90.00

Refinement procedure

Resolution	20.216 - 2.200
R-factor	0.1876
Rwork	0.182
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2GOZ
RMSD bond length	0.005
RMSD bond angle	0.938
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.350	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.030	0.310
Number of reflections	9191
<I/σ(I)>	16.8	3.8
Completeness [%]	96.1	98.2
Redundancy	3.9	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1