3ZD1
STRUCTURE OF THE TWO C-TERMINAL DOMAINS OF COMPLEMENT FACTOR H RELATED PROTEIN 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2011-12-16 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 52.950, 25.180, 95.680 |
| Unit cell angles | 90.00, 93.80, 90.00 |
Refinement procedure
| Resolution | 19.090 - 2.000 |
| R-factor | 0.2121 |
| Rwork | 0.211 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g7i |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.470 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 | 0.260 |
| Number of reflections | 16993 | |
| <I/σ(I)> | 15.2 | 4 |
| Completeness [%] | 96.7 | 86.8 |
| Redundancy | 3.2 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 30% (W/V) PEG 8000, 0.2M AMMONIUM SULPHATE |
