3ZCP
Rabbit muscle glycogen phosphorylase b in complex with N- cyclohexancarbonyl-N-beta-D-glucopyranosyl urea determined at 1.83 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2006-10-06 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 128.677, 128.677, 116.451 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.270 - 1.830 |
R-factor | 0.19198 |
Rwork | 0.191 |
R-free | 0.21109 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qnb |
RMSD bond length | 0.007 |
RMSD bond angle | 0.961 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.860 |
High resolution limit [Å] | 1.830 | 1.830 |
Rmerge | 0.040 | 0.430 |
Number of reflections | 83585 | |
<I/σ(I)> | 23 | 4.3 |
Completeness [%] | 96.7 | 95.9 |
Redundancy | 5.4 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.8 | pH 6.8 |